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Is cytochrome P‐450 scc a transmembrane protein?
Author(s) -
Usanov Sergey A.,
Chernogolov Alexey A.,
Chashchin Vadim L.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81432-n
Subject(s) - cytochrome , cytochrome c , transmembrane protein , cytochrome b , cytosol , transmembrane domain , membrane protein , chemistry , inner mitochondrial membrane , biochemistry , membrane topology , membrane , biology , microbiology and biotechnology , mitochondrion , gene , mitochondrial dna , receptor , enzyme
The topology of cytochrome P‐450 scc in the inner mitochondrial membrane of adrenal cortex has been investigated using monospecific antibodies to cytochrome P‐450 scc and its fragments F 1 (Ile 1 ‐Arg 250 ), F 2 (Asn 257 ‐Ala 481 ) and F 3 (Asn 257 ‐Arg 399 ). Antibodies to F 1 and F 2 were shown to effectively bind to the matrix and cytosolic sides of the inner membrane. Antibodies to F 3 specifically interacted only with the matrix side of the membrane. These data are consistent with a model of molecular organization which shows that cytochrome P‐450 scc is a transmembrane protein, both N‐ and C‐terminal sequences of the cytochrome being able to span the membrane.