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Degradation of crystallins from a psoriatic patient undergoing PUVA therapy
Author(s) -
e Melo Teresa Sa,
de Castro José Cime,
Ribeiro Luisa,
Conte Joao,
Lawrence David,
Bazin Marc,
Santus René
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80975-o
Subject(s) - puva therapy , dermatology , psoriasis , medicine , chemistry
Comparative physico‐chemical and spectroscopic analyses were carried out in human lens proteins obtained from extracts of normal, senile and PUVA cataracts. Mass recovery analysis reveals a large protein concentration loss in the PUVA cataract relative to the normal lens and senile cataract. This protein loss parallels an increase in the degraded polypeptide chains. However, the tryptophan content (2.1of 20 kDa protein subunit) and the apparent fluorescence quantum yield (ϕ f = 0.056) of the tryptophan residues which are believed to be involved in the development of UV‐induced cataracts are unchanged after age‐related alterations and/or in vivo photochemistry associated with psoralen (8MOP) photosensitized reactions.