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Refilling of endothelial calcium stores without bypassing the cytosol
Author(s) -
Lückhoff Andreas,
Busse Rudi
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80519-o
Subject(s) - bradykinin , cytosol , extracellular , chemistry , intracellular , calcium , bradykinin receptor , stimulation , antagonist , biophysics , biochemistry , endocrinology , enzyme , biology , receptor , organic chemistry
The present study was undertaken to define the route of Ca 2+ used for refilling of intracellular Ca 2+ stores in endothelial cells. Ca 2+ stores, after emptying with bradykinin in Ca 2+ free solution and termination of the stimulation with the bradykinin antagonist, Hoe 140, were allowed to refill by addition of Ca 2+ . Refilling was prevented by 2,5‐di( tert ‐butyl)‐1,4‐benzohydroquinone (BuBHQ), an inhibitor of microsomal Ca 2+ sequestration. BuBHQ induced large increases in the cytosolic Ca 2+ concentration during the refilling phase. This finding is not compatible with a model proposing Ca 2+ uptake into the stores directly from the extracellular space but provides evidence for uptake from the cytosolic compartment.