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DNA fingerprints revealing common and divergent human DNA methylation patterns
Author(s) -
Berneman Zwi N.,
Raeymaekers Peter,
Vanhoof Greet,
Van Bockstaele Dirk,
Ramael Marc,
Peetermans Marc E.,
Vandenberghe Antoon,
Van Broeckhoven Christine
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81096-8
Subject(s) - biology , dna profiling , dna methylation , dna , genetics , gene , gene expression
We compared DNA fingerprints of different cell populations from the same individuals, after separate digestion with the isoschizomers Mbo I and Sau 3A. Methylation differences were observed within every individual when comparing fingerprints of Sau 3A‐ with Mbo I‐digested DNA, and of Sau 3A‐digested sperm with somatic DNA. In some cases, differences were also detected between fingerprints of Sau 3A‐digested somatic DNA originating from various cell sources. Methylation patterns common to all cell populations examined, including the germline, were observed with a higher frequency than divergent ones. These ‘common methylations’ are most likely to find their origin during early embryogenesis.