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19 F NMR detection of a fluorine‐labelled enzyme in vivo
Author(s) -
Brindle Kevin,
Williams Simon-Peter,
Boulton Mark
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81073-7
Subject(s) - enzyme , chemistry , in vivo , yeast , saccharomyces cerevisiae , fluorine 19 nmr , fluorine , tryptophan , phosphoglycerate kinase , biochemistry , nuclear magnetic resonance spectroscopy , stereochemistry , organic chemistry , biology , amino acid , microbiology and biotechnology
Phosphoglycerate kinase was fluorine‐labelled in the yeast Saccharomyces cerevisiae by inducing enzyme synthesis in the presence of 5‐fluorotryptophan. 19 F NMR measurements on the intact cells showed two resonances from the two tryptophan residues in the protein. The chemical shifts of these resonances were sensitive to the metabolic status of the cells.