Premium
Production of carrier proteins for insulin‐like growth factors (IGFs) by rat osteoblastic cells Regulation by IGF I and cortisol
Author(s) -
Schmid C.,
Zapf J.,
Froesch E.R.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80556-3
Subject(s) - medicine , endocrinology , in vitro , western blot , cell culture , binding protein , hormone , growth factor , growth hormone , insulin like growth factor binding protein , insulin , chemistry , insulin like growth factor , blot , biology , receptor , biochemistry , gene , genetics
A bone‐derived rat cell line, PyMS, releases IGF I and IGF carrier proteins which are similar to those found in rat serum. Western blot analysis of culture media conditioned by hormone‐treated cells shows that growth hormone and IGF I stimulate and cortisol inhibits production of IGF carrier proteins in vitro. A glycosylated carrier protein species of 49‐42 kDa is closely related to the subunits of the growth hormone‐dependent carrier protein complex found in rat serum. In addition, rhIGF I rapidly induces a 32 kDa, non‐glycosylated IGF‐binding protein whose accumulation is markedly increased by cortisol.