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cDNA and derived amino acid sequence of the hypusine containing protein from Dictyostelium discoideum
Author(s) -
Sandholzer Ute,
Centea-Intemann Mihaela,
Noegel Angelika A.,
Lottspeich Friedrich
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80260-1
Subject(s) - dictyostelium discoideum , cyanogen bromide , biology , amino acid , complementary dna , peptide sequence , biochemistry , microbiology and biotechnology , protein biosynthesis , gene
The eukaryotic translation initiation factor eIF‐4D is the only protein known to contain the unusual amino acid hypusine, a posttranslationally modified lysine. For the production of monoclonal antibodies the hypusine‐containing protein (HP) was isolated from Dictyostelium discoideum . Using these monoclonal antibodies, a full‐length cDNA clone was isolated from a λgt11 library. The D. discoideum HP consists of 169 amino acids and has a molecular mass of 18.3 kDa. It is encoded by a single gene. Tryptic and cyanogen bromide peptides were prepared from the purified protein and sequenced. The hypusine residue is located at amino acid position 65 of the HP. The corresponding mRNA of approx. 0.6 kb is present throughout the life cycle of D. discoideum .