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An enzyme which specifically splits a covalent bond between picornaviral RNA and VPg
Author(s) -
Drygin Yuri,
Shabanov Alexei,
Bogdanov Alexei
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80948-7
Subject(s) - covalent bond , enzyme , spermidine , biochemistry , rna , reagent , chemistry , in vitro , stereochemistry , microbiology and biotechnology , biology , organic chemistry , gene
An enzyme which specifically splits a covalent bond between VPg and picornaviral RNAs (tentatively designated Y‐pUpN PDE) has been partially purified from mouse ascites Krebs II cells. Using substrates labelled in vitro with 125 I‐Bolton‐Hunter reagent and a new assay based on Kieselgel thin‐layer chromatography, several biochemical characteristics of the enzyme have been determined, depending on the pH and on Mg 2+ , K + , spermidine and PEG concentrations, etc. We found that the enzyme does not ‘unlink’ VPg from comoviral RNA. We suggest that Y‐pUpN PDE represents a new class of enzymes.