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Derivation of the sequence of the signal peptide in human C4b‐binding protein and interspecies cross‐hybridisation of the C4bp cDNA sequence
Author(s) -
Lintin S.J.,
Lewin A.R.,
Reid K.B.M.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80763-4
Subject(s) - complementary dna , biology , coding region , peptide sequence , microbiology and biotechnology , genetics , sequence (biology) , signal peptide , genomic dna , sequence analysis , nucleic acid sequence , protein sequencing , conserved sequence , southern blot , dna , gene
A 5′ cDNA clone coding for human C4b‐binding protein (C4bp) was isolated, characterised and sequenced to complete the cDNA sequence coding for residues 1–32 thus confirming the protein sequence data of Chung et al. [(1985) Biochem. J. 230, 133–141]. The sequence extended to allow derivation of the putative leader peptide sequence which was 32 residues in length and showed a high of hydrophobicity typical of other documented leader sequences. Cross hybridisation was detected between the human C4bp cDNA probes and genomic DNA isolated from various species on Southern blots suggesting that genomic sequence homologous to that coding for C4bp has been conserved during evolution.