Premium
Biotransformation of aromatic compounds Monitoring fluorinated analogues by NMR
Author(s) -
Cass Anthony E.G.,
Ribbons Douglas W.,
Rossiter John T.,
Williams Steve R.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80845-1
Subject(s) - biotransformation , chemistry , nuclear magnetic resonance spectroscopy , fluorine 19 nmr , metabolic pathway , in vivo , carbon 13 nmr , biochemical engineering , organic chemistry , metabolism , biochemistry , biology , enzyme , microbiology and biotechnology , engineering
The results presented here illustrate the power of NMR in the non‐invasive analysis of microbial transformations. Whilst the definitive identification of products requires purification and full structural elucidation, NMR can provide rapid insights into the nature of these reactions and their regulation in vivo. In addition, once the products have been identified NMR methods allow rapid assessment of the effects of genetic and physiological manipulation, and on competing metabolic fluxes with mixed substrates and branched pathways.