Premium
The role of adenine nucleotide translocators in regulation of oxidative phosphorylation in heart mitochondria
Author(s) -
Kholodenko B.,
Z̆ilinskie·e V.,
Borutait·e V.,
Ivanovien·e L.,
Toleikis A.,
Pras̆kevic̆ius A.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80298-3
Subject(s) - oxidative phosphorylation , respiration , respiration rate , mitochondrion , adenine nucleotide , biology , phosphorylation , ant , biochemistry , bioenergetics , atp synthase , microbiology and biotechnology , nucleotide , enzyme , anatomy , ecology , gene
The regulative role of adenine nucleotide translocators (ANTs) in oxidative phosphorylation has been estimated by the titration of respiration of isolated rabbit heart mitochondria with carboxyatractyloside in the presence of a non‐rate limiting creatine phosphokinase ADP‐regenerating system. It has been established that the respiration rate is not controlled by ANTs in the two extreme states, state 3 and state 4. On the other hand, at an intermediate respiration rate (30–70% of the state 3 respiration, which roughly corresponds to that under physiological conditions) the ANT control coefficient had a value of 0.62–0.75. Thus, ANTs seem to play a key role in the regulation of oxidative phosphorylation.