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Inhibition of topoisomerase I by antibodies in sera from scleroderma patients
Author(s) -
Scott Samuels D.,
Tojo Takeshi,
Homma Mitsuo,
Shimizu Nobuyoshi
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81117-6
Subject(s) - scleroderma (fungus) , antibody , topoisomerase , chemistry , medicine , microbiology and biotechnology , immunology , biochemistry , biology , enzyme , inoculation
Purified type I topoisomerase from calf thymus as well as nuclear and cytoplasmic extracts from EGF‐stimulated human and mouse fibroblasts in cell culture efficiently convert supercoiled plasmid DNA to the relaxed form. The purified IgG fraction from the sera of Japanese patients with the rheumatic disease scleroderma were shown to inhibit this relaxation activity. Thus, these patients likely produce autoantibodies to topoisomerase I. In addition, the human, bovine and murine enzymes share antigenic determinants recognized by the antisera.