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Induction of cell surface insulin antigenicity in fibroblasts transfected with islet genomic DNA
Author(s) -
Klein Benjamin Y.,
Nesher Rafael,
Cerasi Erol
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80925-5
Subject(s) - transfection , microbiology and biotechnology , genomic dna , dna , cell , insulinoma , biology , cell culture , antigenicity , insulin , antigen , biochemistry , genetics , endocrinology
Ltk − cells were transfected with total genomic DNA obtained from rat islets or from insulinoma cells mixed with DNA tagged with fluorescent dye. Flyorescence‐activated cell sorter (FACS) was used for initial selection of successful transfectants, monitoring fluorescent DNA incorporated into the cell. For subsequent selections the cells were treated with anti‐insulin antiserum labeled with fluorescent dye and selected by FACS. B cell DNA, tiut not DNA from murine leukemic cells, induced the appearance of cell surface insulin antigenicity in fibroblasts. This phenotypic expression was transient. Together with our previous demonstration of the induction of insulin secreation in Ltk − cells [(1986) Biochem. Biophys. Res. Commun. 136, 638‐644], these results indicate that B cell specific characteristics can be transfected to non‐endocrine cells by genomic DNA transfection.