A simple procedure to determine Ca 2+  in oxygen‐evolving preparations from  Synechococcus  sp | Zendy

Kashino Yasuhiro | Zendy; Satoh Kazuhiko | Zendy; Katoh Sakae | Zendy

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A simple procedure to determine Ca 2+ in oxygen‐evolving preparations from Synechococcus sp

Author(s) -

Kashino Yasuhiro,

Satoh Kazuhiko,

Katoh Sakae

Publication year - 1986

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/0014-5793(86)80884-5

Subject(s) - chemistry , suspension (topology) , oxygen , synechococcus , chelation , metal , particle size , particle (ecology) , chromatography , nuclear chemistry , inorganic chemistry , cyanobacteria , organic chemistry , bacteria , geology , paleontology , oceanography , mathematics , homotopy , pure mathematics

A simple procedure to determine Ca 2+ bound to low‐ and high‐affinity sites of Synechococcus oxygen‐evolving particles was developed. The method consists of determination of Ca 2+ in the particle suspensions with and without treatment with a chelating resin, Chelex 100, to remove the metal cations contaminating the suspension medium as well as those weakly bound to the particles. It was found that the particles contain one tightly bound Ca 2+ per PS II reaction center which cannot be extracted with Chelex 100 and a larger amount of weakly associated and resin‐extractable Ca 2+ .

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