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Leukotriene B 4 stimulation of phosphatidylinositol turnover in macrophages and inhibition by pertussis toxin
Author(s) -
Holian Andrij
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80562-2
Subject(s) - pertussis toxin , phosphatidylinositol , stimulation , bordetella pertussis , toxin , leukotriene c4 , leukotriene , chemistry , microbiology and biotechnology , immunology , g protein , biology , receptor , biochemistry , endocrinology , signal transduction , bacteria , genetics , asthma
The influence of leukotriene B 4 (LTB 4 ) on phosphatidylinositol (PI) cycle activity was investigated in the guinea pig alveolar macrophage. In contrast to the observation reported in leukocytes [(1984) Proc. Natl. Acad. Sci. USA 81, 5966‐5969], LTB 4 was found to stimulate PI cycle activity in the macrophage (transient decrease in phosphatidylinositol 4,5‐bisphosphate and phosphatidylinositol 4‐phosphate, transient elevation of 1,2‐diacylglycerol and elevation of phosphatidic acid) similar to the stimulation of the PI cycle by the chemotactic formyi peptides. Preincubation of macrophages with 1 μ/ml of pertussis toxin, for 1 h at 37°C, blocked LTB 4 and formyl peptide stimulated O 2 − production and PI cycle activity. Therefore, both stimulants are proposed to act through the same coupling protein to activate phospholipase C in the early stages of macrophage activation.