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Acetylcholine stimulates phosphatidylinositol turnover at nicotinic receptors of cultured myotubes
Author(s) -
Adamo Sergio,
Zani Bianca Maria,
Nervi Clara,
Senni Maria Immacolata,
Molmaro Mario,
Eusebi Fabrizio
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80449-x
Subject(s) - phosphatidylinositol , chemistry , myogenesis , acetylcholine receptor , nicotinic agonist , ganglion type nicotinic receptor , muscarinic acetylcholine receptor m5 , acetylcholine , alpha 4 beta 2 nicotinic receptor , receptor , microbiology and biotechnology , endocrinology , biochemistry , muscarinic acetylcholine receptor m3 , nicotinic acetylcholine receptor , biology , signal transduction , in vitro
Acetylcholine treatment of [ 3 H]inositol pre‐labelled cultured chick embryo myotubes results in the stimulation of phosphatidylinositol breakdown, as shown by the measurement of inositol‐1‐phosphate accumulating in the presence of lithium. The described effect is dependent on agonist concentration and incubation time, and is inhibited by tubocurarine and α‐bungarotoxin. The activation of phosphatidylinositol breakdown by acetylcholine at extrajunctional nicotinic receptors is likely to be involved in the modulation of the functional activity of the receptor.