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Demonstration of chondroitin sulfates degrading endo‐β‐glucuronidase activity in rabbit liver
Author(s) -
Takagaki Keiichi,
Nakamura Toshiya,
Majima Mitsuo,
Endo Masahiko
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80274-x
Subject(s) - chondroitin sulfate , chemistry , glucuronic acid , chondroitin , incubation , chromatography , sulfate , sephadex , biochemistry , sodium borohydride , glycosaminoglycan , enzyme , polysaccharide , organic chemistry , catalysis
Reduced chondroitin sulfate was incubated with rabbit liver extracts followed by reduction once more with sodium [ 3 H]borohydride, and then passed through a Sephadex G‐100 column. Chondroitin sulfate obtained from the incubation medium at pH 4 was only slightly depolymerized and was highly radioactive. Paper Chromatographic analyses showed that glucuronic acid residues became exposed at the reducing terminal of chondroitin sulfate after incubation with the liver extracts. These results suggest that endo‐β‐glucuronidase activity which degrades chondroitin sulfate is present in the rabbit liver.