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In vivo proton NMR spectroscopy
Author(s) -
Doddrell David M.,
Kathryn M Nicholls,
Sanders Jeremy K.M.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)81371-x
Subject(s) - nuclear magnetic resonance spectroscopy , formaldehyde , in vivo , chemistry , proton , pulse sequence , proton nmr , nuclear magnetic resonance , magnetization transfer , metabolism , spectroscopy , analytical chemistry (journal) , biochemistry , stereochemistry , chromatography , biology , physics , medicine , microbiology and biotechnology , radiology , quantum mechanics , magnetic resonance imaging
The metabolism of [ 13 C]formaldehyde in E.coli has been observed by proton NMR; the reverse‐POMMIE pulse sequence, which saturates all 1 H signals and then transfer magnetisation from 13 C to 1 H, suppresses water by 10 5 ‐fold, allowing observation of metabolism at the millimolar level