Three sequence‐specific endonucleases from  Escherichia coli  RFL47 | Zendy

Janulaitis A. | Zendy; Petrušyté M. | Zendy; Butkus V. | Zendy

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Three sequence‐specific endonucleases from Escherichia coli RFL47

Author(s) -

Janulaitis A.,

Petrušyté M.,

Butkus V.

Publication year - 1983

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/0014-5793(83)81010-2

Subject(s) - isoschizomer , escherichia coli , restriction enzyme , recognition sequence , palindromic sequence , biology , genetics , microbiology and biotechnology , mutant , dna , gene , palindrome , chemistry , genome , gene expression , dna methylation

The characterization of the new restriction enzyme Eco 47III recognizing a hexanucleotide palindromic sequence 5'AGC ↓ GCT and cleaving, as indicated by the arrow, is reported. It was isolated from Escherichia coli strain RFL47. Another two specific endonuclease Eco 47I (isoschizomer of Ava II) and Eco 47II (isoschizomer of Asu I) were also found in this strain. There are two Eco 47III recognition sites on λ DNA at 20997 and 37060 basepairs. The central Eco 47III fragment can be replaced by a cloned fragment in λ vector mutant in t R2 gene; i.e., λ gt

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