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Cloning and DNA sequence of the genes for two bacteriophage T5 tRNAs Ser
Author(s) -
Kryukov V.M.,
Ksenzenko V.N.,
Kaliman A.V.,
Bayev A.A.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80690-5
Subject(s) - transfer rna , dna , gene , bacteriophage , plasmid , cloning (programming) , genetic code , nucleic acid sequence , genetics , sequence (biology) , nucleotide , molecular cloning , biology , dna sequencing , microbiology and biotechnology , rna , peptide sequence , escherichia coli , computer science , programming language
Bacteriophage T5 Bgl II/ Hin dIII DNA fragment (803 basepairs), containing the genes for 2 tRNAs and 2 RNAs with unknown functions, was cloned in the plasmid pBR322. The analysis of DNA sequence indicates that tRNA genes code isoacceptor tRNAs Ser (tRNA Ser 1 and tRNA Ser 2 ) with anticodons UGA and GGA, respectively. The main unusual structural feature of these tRNAs is the presence of extra non‐basepaired nucleotides in the joinings of stem ‘b’ with stems ‘a’ and ‘c’.
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