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Binding specificities of the lectins from Helix pomatia , soybean and peanut against different glycosphingolipids in liposome membranes
Author(s) -
Månsson Jan-Eric,
Olofsson Sigvard
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80506-7
Subject(s) - helix pomatia , peanut agglutinin , ganglioside , soybean agglutinin , biochemistry , sialic acid , lectin , agglutinin , liposome , chemistry , galactose , biology , snail , ecology
The binding specificities of the lectins from Helix pomatia , soybean and peanut against glycosphingolipids containing β‐linked terminal D‐galactose and N‐acetyl‐D‐galactosamine and the role of sialic acid as a modulator of the binding specificity were investigated. The test system used consisted of liposomes containing the glycosphingolipids and lectins coupled to gel columns. Of the investigated glycosphingolipids only ganglioside GM2 bound to soybean agglutinin while Helix pomatia agglutinin was found to bind only GA2. Peanut agglutinin showed good affinity both for ganglioside GM1 and its asialoderivative GA1.