Premium
Evidence for a glycolate transporter in the envelope of pea chloroplasts
Author(s) -
Howitz Konrad T.,
McCarty Richard E.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80178-1
Subject(s) - chloroplast , photorespiration , chloroplast membrane , glyoxylate cycle , chemistry , transporter , biochemistry , differential centrifugation , biophysics , biology , metabolism , photosynthesis , thylakoid , gene
Glycolate excretion by chloroplasts is essential to the process of photorespiration. Previous transport studies on intact chloroplasts have failed to find evidence for carrier‐mediated transport of glycolate. The rate of glycolate uptake, when measured by a rapid silicone oil centrifugation method, saturates at high glycolate concentrations. This rapid glycolate uptake is inhibited by pretreatment of the chloroplasts with N ‐ethylmaleimide, an inhibition prevented by glycolate. Glyoxylate and glycerate inhibit glycolate uptake when present in the assay medium. These results suggest the existence of a glycolate transporter in the chloroplast envelope.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom