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Influence of temperature and denaturing agents on the structural stability of calmodulin
Author(s) -
Guerini Danilo,
Krebs Joachim
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80029-5
Subject(s) - calmodulin , chemistry , structural integrity , calcium , biophysics , nuclear magnetic resonance spectroscopy , biochemistry , biology , stereochemistry , organic chemistry , structural engineering , engineering
The structural stability of calmodulin was studied by 1 H‐nuclear magnetic resonance spectroscopy under different denaturing conditions. The presence of Ca 2+ stabilizes the structural properties of the native protein. In the absence of calcium the structural integrity of calmodulin can easily be affected by elevated temperatures or by high concentrations of denaturing agents. The unfolding process under various denaturing conditions is reversible underlining the high degree of structural flexibility of this protein.