Open AccessEvaluation of the in vitro and in vivo genotoxicity of a Dioscorea Rhizome water extract
Author(s) -
Seung-Beom Cha,
Seong-Sook Kim,
Jeong-Ja Oh,
Woo-Joo Lee,
Si-Whan Song,
JeOh Lim,
Jong-Choon Kim
Publication year - 2021
Publication title -
toxicological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.872
H-Index - 30
eISSN - 2234-2753
pISSN - 1976-8257
DOI - 10.1007/s43188-020-00077-8
Subject(s) - genotoxicity , micronucleus test , ames test , in vivo , micronucleus , clastogen , chromosome aberration , biology , gene mutation , pharmacology , in vitro , salmonella , chemistry , genetics , chromosome , mutation , toxicity , gene , bacteria , organic chemistry
Dioscorea Rhizome is commonly used in traditional herbal medicines for the treatment of diabetes, hyperthyroidism, liver damage, neuropathy, and asthma. Here, we investigated the genotoxicity potential of D. Rhizome water extract (DRWE) using three standard battery systems in accordance with the test guidelines of the Organisation for Economic Cooperation and Development and Ministry of Food and Drug Safety as well as the principles of Good Laboratory Practice. A bacterial reverse mutation test (Ames test) was performed using the direct plate incorporation method in the presence or absence of a metabolic activation system (S9 mixture). The tester strains used included four histidine auxotrophic strains of Salmonella typhimurium , TA100, TA1535, TA98, and TA1537, along with a tryptophan auxotrophic strain of Escherichia coli , WP2 uvr A. An in vitro chromosome aberration test was performed using CHL/IU cells originally derived from the lung of a female Chinese hamster in the presence or absence of the S9 mixture. An in vivo mouse bone marrow micronucleus test was performed using male ICR mice. The micronucleus was confirmed after observation of the micro-nucleated polychromatic. The Ames test showed that DRWE did not induce gene mutations at any dose level in any of the tested strains. Additionally, DRWE did not result in any chromosomal aberrations specified in the in vitro chromosomal aberration and in vivo micronucleus tests. These results showed that DRWE exhibited neither mutagenic nor clastogenic potential in either the in vitro or in vivo test systems.