Open AccessBackbone and side-chain chemical shift assignments of p50 subunit of NF-κB transcription factor
Author(s) -
Amrinder Singh,
H. Jane Dyson
Publication year - 2020
Publication title -
biomolecular nmr assignments
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.359
H-Index - 16
eISSN - 1874-2718
pISSN - 1874-270X
DOI - 10.1007/s12104-020-09978-z
Subject(s) - transcription factor , enhancer , p50 , protein subunit , transcription (linguistics) , dna , chemistry , promoter , genetics , electrophoretic mobility shift assay , gene , side chain , microbiology and biotechnology , biology , gene expression , linguistics , philosophy , organic chemistry , polymer
Nuclear Factor κB (NF-κB) is a family of five related transcription factors that recognize a κB DNA element on the promoter and enhancer regions of target genes and modulate their expression. Here we report a complete set of 1 H, 13 C, 15 N backbone and side chain resonance assignments for the p50 DNA binding and dimerization domains of the p50 homodimer form of the NF-κB transcription factor. The chemical shift data constitute a first step towards understanding the mechanism of interaction of the p50 homodimer with DNA.