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Identification and estimation of tocotrienols in the annatto lipid fraction by gas chromatography‐mass spectrometry
Author(s) -
Frega N.,
Mozzon M.,
Bocci F.
Publication year - 1998
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-998-0323-1
Subject(s) - tocotrienol , chromatography , chemistry , fraction (chemistry) , gas chromatography , mass spectrometry , hexane , gas chromatography–mass spectrometry , tocopherol , antioxidant , thin layer chromatography , vitamin e , organic chemistry
Abstract The lipid fraction of annatto ( Bixa orellana L.) seeds was extracted with a Soxhlet apparatus with n ‐hexane and isolated by thin‐layer chromatography. The fatty‐soluble antioxidant fraction contained only tocotrienols, mainly δ‐tocotrienol, but no tocopherols. The presence of tocotrienols was confirmed by gas chromatography‐mass spectrometry. The quantities of δ‐tocotrienol were 140–147 mg/100 g dry seeds and 5.2–5.5% wt/wt of lipid extract, determined by gas chromatography and high‐performance liquid chromatography, respectively. Currently no vegetable species seems to contain comparable concentrations of δ‐tocotrienol.