Premium
Improved Estimation of Oil, Linoleic and Oleic Acid and Seed Hull Fractions in Safflower by NIRS
Author(s) -
Rudolphi Sabine,
Becker Heiko C.,
Schierholt Antje,
WitzkeEhbrecht Sabine
Publication year - 2012
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-011-1920-y
Subject(s) - carthamus , linoleic acid , oleic acid , fraction (chemistry) , meal , near infrared reflectance spectroscopy , yield (engineering) , agronomy , chemistry , horticulture , biology , botany , food science , fatty acid , chromatography , near infrared spectroscopy , materials science , biochemistry , medicine , neuroscience , traditional medicine , metallurgy
Abstract Near‐infrared reflectance spectroscopy (NIRS) of intact seeds allows the non‐destructive estimation of seed quality parameters which is highly desirable in plant breeding. Together with yield, oil content and quality, a main aim in safflower ( Carthamus tinctorius L.) breeding is the selection of genotypes with a low percentage of empty seeds even under cooler climates. We developed NIRS calibrations for seed oil content, oleic and linoleic acid content, the seed hull fraction and the percentage of empty seeds using seed meal and intact seeds. For the different calibrations 108–534 samples from a safflower breeding program with lines adapted to German conditions, were analyzed with reference analyses (Soxhlet, gas chromatography), and scanned by NIRS as intact seeds and seed meal. Calibration equations were developed and tested through cross validation. The coefficient of determination of the calibration ( R 2 ) for intact seeds ranged from 0.91(oil content), 0.90 (seed hull fraction), 0.84 (empty seeds), 0.73 (linoleic acid) to 0.68 (oleic acid). The coefficient of determination of the cross validation was higher for seed meal than for intact seeds except for the parameter seed hull fraction. The results show that NIRS calibrations are applicable in safflower breeding programs for a fast screening.