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Metabolism of trans fatty acids by hepatocytes
Author(s) -
Guzmán Manuel,
Klein Wil,
Gómez del Pulgar Teresa,
Geelen Math J. H.
Publication year - 1999
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-999-0376-6
Subject(s) - elaidic acid , oleic acid , peroxisome , biochemistry , metabolism , carnitine palmitoyltransferase i , fatty acid , chemistry , substrate (aquarium) , beta oxidation , carnitine , malonyl coa , fatty acid metabolism , biology , linoleic acid , ecology , gene
Abstract The present work was undertaken to study the metabolism of fatty acids with trans double bonds by rat hepatocytes. In liver mitochondria, elaidoyl‐CoA was a poorer substrate for carnitine palmitoyltransferase I (CPT‐I) than oleoyl‐CoA. Likewise, incubation, of hepatocytes with oleic acid produced a more pronounced stimulation of CPT‐I than incubation with trans fatty acids. This was not due to a differential effect of cis and trans fatty acids on acetyl‐CoA carboxylase (ACC) activity and malonyl‐CoA levels. Elaidic acid was metabolized by hepatocytes at a higher rate than oleic acid. Surprisingly, compared to oleic acid, elaidic acid was a better substrate for mitochondrial and, especially, peroxisomal oxidation, but a poorer substrate for cellular and very low density lipoprotein triacylglycerol synthesis. Results thus show that trans fatty acids are preferentially oxidized by hepatic peroxisomes, and that the ACC/malonyl‐CoA/CPT‐I system for coordinate control of fatty acid metabolism is not responsible for the distinct hepatic utilization of cis and trans fatty acids.