Open AccessGene expression analysis of ovine prepubertal testicular tissue vitrified with a novel cryodevice (E.Vit)
Author(s) -
Daniela Bebbere,
Sara Pinna,
Stefano Nieddu,
Dity Natan,
Amir Arav,
Sergio Ledda
Publication year - 2019
Publication title -
journal of assisted reproduction and genetics
Language(s) - English
Resource type - Journals
eISSN - 1573-7330
pISSN - 1058-0468
DOI - 10.1007/s10815-019-01559-x
Subject(s) - cryopreservation , andrology , trypan blue , biology , somatic cell , seminiferous tubule , cd44 , germ cell , in vitro maturation , cell , spermatogenesis , embryo , microbiology and biotechnology , oocyte , gene , medicine , sertoli cell , genetics
Testicular tissue cryopreservation prior to gonadotoxic therapies is a method to preserve fertility in children. However, the technique still requires development, especially when the tissue is immature and rather susceptible to stress derived from in vitro manipulation. This study aimed to investigate the effects of vitrification with a new cryodevice (E.Vit) on cell membrane integrity and gene expression of prepubertal testicular tissue in the ovine model.