Open AccessNISES-AnPe-428 cell line derived from the Chinese oak silkworm Antheraea pernyi is permissive for multiple nucleopolyhedrovirus species from insects of four different families
Author(s) -
Shinya Isobe,
Ayaka Ota,
Shiori Takata,
Rina Hamajima,
Shizuka Makino,
Jun Kobayashi,
Michihiro Kobayashi,
Motoko Ikeda
Publication year - 2021
Publication title -
cytotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.488
H-Index - 62
eISSN - 1573-0778
pISSN - 0920-9069
DOI - 10.1007/s10616-021-00485-0
Subject(s) - antheraea pernyi , biology , autographa californica , virology , permissiveness , bombyx mori , viral replication , virus , spodoptera , genetics , gene , recombinant dna
The cell line NISES-AnPe-428 (AnPe), derived from the Chinese oak silkworm Antheraea pernyi , was characterized for its permissiveness and productivity for six different nucleopolyhedrovirus (NPV) species. These NPVs included homologous Antheraea pernyi NPV (AnpeNPV) and heterologous Autographa californica multiple NPV (AcMNPV), Bombyx mori NPV (BmNPV), Hyphantria cunea MNPV (HycuMNPV), Spodoptera exigua MNPV (SeMNPV), and Lymantria dispar MNPV (LdMNPV), representing viruses that had been isolated from insect species belonging to five different families ( Saturniidae , Noctuidae , Bombycidae , Arctiidae , and Lymantriidae ). We found that AnPe cells supported productive replication of AnpeNPV, AcMNPV, BmNPV, HycuMNPV, and SeMNPV to varying degrees. Upon infection with SeMNPV, a subset of AnPe cell population in the culture underwent apoptosis, while remaining cells produced limited amounts of progeny virions and polyhedra. AnPe cells were refractory to LdMNPV infection and failed to support replication of viral DNA, indicating that viral replication was restricted at or prior to the step of viral DNA replication. These results indicated that AnPe cells have the potential to provide excellent systems for studying the molecular mechanisms underlying cellular permissiveness for NPV replication and host-range determination of NPVs.