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Coloring conditions of thiobarbituric acid test for detecting lipid hydroperoxides
Author(s) -
Asakawa T.,
Matsushita S.
Publication year - 1980
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02540959
Subject(s) - autoxidation , thiobarbituric acid , chemistry , clinical chemistry , glycine , lipidology , hydrochloric acid , fatty acid , antioxidant , organic chemistry , chromatography , food science , lipid peroxidation , biochemistry , amino acid
Abstract The coloring reaction of the thiobarbituric acid test for hydroperoxides was completely inhibited by the addition of EDTA. Therefore, it was necessary to add a metal salt to the reaction mixture to complete the reaction and also to add an antioxidant to prevent autoxidation when unoxidized unsaturated fatty acids co‐exist. The optimal pH of the reaction was found at 3.6 using glycine‐hydrochloric acid buffer.