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Subcellular distribution of the cytochrome P‐450 complex and glutathione peroxidase activity in vitamin E and essential fatty acid deficiency
Author(s) -
Jensen Gunde Egeskov,
Clausen Jørgen
Publication year - 1981
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535688
Subject(s) - microsome , glutathione peroxidase , mitochondrion , inner mitochondrial membrane , chemistry , clinical chemistry , mitochondrial matrix , vitamin e , vitamin , biochemistry , cytochrome , antioxidant , medicine , glutathione , enzyme , cytosol
Abstract Glutathione peroxidase (EC 1.11.1.9) (GSHPx) and P‐450 activity were measured in hepatic mitochondrial and microsomal fractions from rats deficient in vitamin E and/or essential fatty acids (EFA). The data were compared to corresponding normal values. GSHPx was significantly decreased in the mitochondrial matrix from animals in all 3 deficiency states. In vitamin E deficiency, a nonsignificant decreased GSHPx activity was found in mitochondrial membranes. Opposite to these findings, GSHPx was significantly increased in mitochondrial membranes of EFA‐deficient animals. In combined EFA and vitamin E deficiency, the mitochondrial membrane GSHPx activity was only insignificantly increased. The P‐450 complex activity was not detectable in the mitochondrial matrix. In mitochondrial membranes and microsomes, the P‐450 complex activity changed parallel to the GSHPx activity.