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Preparation of 3 H‐phosphatidylmyoinositol with Kloeckera brevis
Author(s) -
Prottey Colin,
Seidman Michael M.,
Ballou Clinton E.
Publication year - 1970
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02531309
Subject(s) - lipidology , inositol , chemistry , biochemistry , chromatography , clinical chemistry , moiety , enzyme , stereochemistry , receptor
The growth of Kloeckera brevis in the presence of uniformly tritiated myoinositol is described. The major radioactive lipid isolated from the cells is 3 H‐phosphatidylmyoinositol, labeled exclusively in the myoinositol moiety. Chromatographic methods for purifying 3 H‐phosphatidylmyoinositol, free from other labeled lipids, are described, and the use of this labeled lipid as a precursor in studying enzymatic synthesis of complex inositol lipids is demonstrated.
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