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A cell cycle study of human mammary epithelial cells.
Author(s) -
Larsson Olle,
Blegen Harald,
Wejde Johan,
Zetterberg Anders
Publication year - 1993
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1993.1100
Subject(s) - cell growth , biology , doubling time , cell cycle , population , growth factor , cell culture , microbiology and biotechnology , cell , chemically defined medium , endocrinology , medicine , biochemistry , in vitro , receptor , genetics , environmental health
Abstract The growth regulation of human mammary epithelial cells (HMEC) cultured in a growth factor/hormone‐enriched (e.g. EGF, insulin) medium with bovine pituitary extract as the only undefined supplement was studied. The doubling times of the cultures, in which the cells appear in colonies, was 55‐72 h, and a considerable intercolonial heterogenecity in proliferative activity could be demonstrated. However, every colony, irrespective of the size of the growth fraction, comprised a sub‐population of rapidly growing cells which had a mean generation time of approximately 22 h. When insulin was removed from the culture medium, HMEC proliferation was inhibited. This growth inhibition was shown to be a result of a cell cycle‐specific block.