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The development of an enzyme‐linked immunosorbent assay for the determination of cortisol binding globulin.
Author(s) -
Rijswijk A. W.,
Morgenthal J. C.,
Taljaard J. J. F.
Publication year - 1993
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1993.1076
Subject(s) - globulin , transcortin , enzyme , chemistry , endocrinology , medicine , biochemistry , biology
Abstract A simple, reliable and reproducible enzyme‐linked immunosorbent assay (ELISA) using polyclonal antibodies for human cortisol binding globulin (CBG) has been developed. The sensitivity of the ELISA (1.20 fmol CBG/well) compared favourably with the sensitivity of other immunoassays. The excellent agreement (r= 0.98) seen between the present study and a binding assay indicates that the polyclonal antibodies used recognize only intact steroid‐binding CBG. The intra‐ and inter‐assay coefficient of variation (4.0% and 7.1% respectively) compared favourably with those reported by other authors.