The development of an enzyme‐linked immunosorbent assay for the determination of cortisol binding globulin. | Zendy

Rijswijk A. W. | Zendy; Morgenthal J. C. | Zendy; Taljaard J. J. F. | Zendy

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The development of an enzyme‐linked immunosorbent assay for the determination of cortisol binding globulin.

Author(s) -

Rijswijk A. W.,

Morgenthal J. C.,

Taljaard J. J. F.

Publication year - 1993

Publication title -

cell biology international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.932

H-Index - 77

eISSN - 1095-8355

pISSN - 1065-6995

DOI - 10.1006/cbir.1993.1076

Subject(s) - globulin , transcortin , enzyme , chemistry , endocrinology , medicine , biochemistry , biology

A simple, reliable and reproducible enzyme‐linked immunosorbent assay (ELISA) using polyclonal antibodies for human cortisol binding globulin (CBG) has been developed. The sensitivity of the ELISA (1.20 fmol CBG/well) compared favourably with the sensitivity of other immunoassays. The excellent agreement (r= 0.98) seen between the present study and a binding assay indicates that the polyclonal antibodies used recognize only intact steroid‐binding CBG. The intra‐ and inter‐assay coefficient of variation (4.0% and 7.1% respectively) compared favourably with those reported by other authors.

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