Gene regulation in response to protein disulphide isomerase deficiency | Zendy

Nørgaard Per | Zendy; Tachibana Christine | Zendy; Bruun Anette W. | Zendy; Winther Jakob R. | Zendy

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Gene regulation in response to protein disulphide isomerase deficiency

Author(s) -

Nørgaard Per,

Tachibana Christine,

Bruun Anette W.,

Winther Jakob R.

Publication year - 2003

Publication title -

yeast

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.923

H-Index - 102

eISSN - 1097-0061

pISSN - 0749-503X

DOI - 10.1002/yea.978

Subject(s) - biology , endoplasmic reticulum , gene , isomerase , protein disulfide isomerase , promoter , unfolded protein response , yeast , microbiology and biotechnology , glucose 6 phosphate isomerase , genetics , gene expression , enzyme , biochemistry

We have examined the activities of promoters of a number of yeast genes encoding resident endoplasmic reticulum proteins, and found increased expression in a strain with severe protein disulphide isomerase deficiency. Serial deletion in the promoter of the MPD1 gene, which encodes a PDI1 ‐homologue, revealed a c is‐acting e lement r esponding to deficiency of p rotein disulphide isomerase activity (designated CERP). The presence of the sequence element is necessary and sufficient for the upregulation in response to disulphide isomerase deficiency, as measured by a minimal promoter containing the CERP element. The sequence (GACACG) does not resemble the unfolded protein response element. It is present in the upstream regions of the MPD1, MPD2, KAR2, PDI1 and ERO1 genes. Copyright © 2003 John Wiley & Sons, Ltd.

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