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Sequence of the novel essential gene YJU2 and two flanking reading frames located within a 3.2 kb Eco RI fragment from chromosome X of Saccharomyces cerevisiae
Author(s) -
Forrová Helena,
Kolarov Jordan,
Ghislain Michel,
Goffeau André
Publication year - 1992
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320080509
Subject(s) - biology , orfs , genetics , gene , open reading frame , nucleic acid sequence , coding region , saccharomyces cerevisiae , microbiology and biotechnology , chromosome , peptide sequence
Abstract We have isolated and sequenced a novel gene from Saccharomyces cerevisiae coding for an essential protein of unknown function. The gene called YJU2 was borne on a chromosome X fragments shown by hybridization to intact S. cerevisiae chromosomal DNA fractionated by orthogonal pulsed field electrophoresis. Northern hybridization analysis indicated that the 0.8‐kb transcript of YJU2 is expressed in exponential‐phase cells grown in rich medium (data not shown). Figure 1 shows the nucleotide and deduced amino‐acid sequences of the 834‐bp coding region as well as the nucleotide sequences of the 5′ upstream region and of the 3′ downstream region, together with the flanking neighbouring open reading frames (ORFs), YJU1 and YJU3 .