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Phylogenetic relationships among species of Saccharomyces , Schizosaccharomyces , Debaryomyces and Schwanniomyces determined from partial ribosomal RNA sequences
Author(s) -
Kurtzman Cletus P.,
Robnett Christie J.
Publication year - 1991
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320070107
Subject(s) - biology , schizosaccharomyces pombe , phylogenetic tree , ribosomal rna , saccharomyces , genetics , schizosaccharomyces , phylogenetics , saccharomyces cerevisiae , yeast , gene
Abstract Species of the genera Saccharomyces , Schizosaccharomyces , Debaryomyces and Schwanniomyces were compared from their extent of divergence in three regions from small (18S) and large (25S) subunit ribosomal RNAs comprising a total of 900 nucleotides. With the exception of the closely related Saccharomyces bayanus and S. pastorianus , which appear to have identical sequences, all other species could be distinguished by nucleotide differences in a variable region of the large subunit, and genus‐specific nucleotides were discernible in all three regions. The taxon D. tamarii differed markedly from other species and is excluded from Debaryomyces . By contrast, Schwanniomyces occidentalis showed few nucleotide differences with Debaryomyces spp. and its transfer to Debaryomyces is proposed. Schizosaccharomyces proved to be somewhat more divergent than Saccharomyces and Debaryomyces , but species differences appear insufficient for dividing the genus. Some of the factors influencing estimates of phylogenetic distances from rRNA sequences are discussed.