Open AccessIn Vitro T ‐Cell Generation From Adult, Embryonic, and Induced Pluripotent Stem Cells: Many Roads to One Destination
Author(s) -
Smith Michelle J.,
Webber Beau R.,
Mohtashami Mahmood,
Stefanski Heather E.,
Zún˜igaPflücker Juan Carlos,
Blazar Bruce R.
Publication year - 2015
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.2115
Subject(s) - biology , induced pluripotent stem cell , embryonic stem cell , microbiology and biotechnology , stem cell , progenitor cell , haematopoiesis , cellular differentiation , in vitro , immunology , genetics , gene
Abstract T lymphocytes are critical mediators of the adaptive immune system and have the capacity to serve as therapeutic agents in the areas of transplant and cancer immunotherapy. While T cells can be isolated and expanded from patients, T cells derived in vitro from both hematopoietic stem/progenitor cells (HSPCs) and human pluripotent stem cells (hPSCs) offer great potential advantages in generating a self‐renewing source of T cells that can be readily genetically modified. T‐cell differentiation in vivo is a complex process requiring tightly regulated signals; providing the correct signals in vitro to induce T‐cell lineage commitment followed by their development into mature, functional, single positive T cells, is similarly complex. In this review, we discuss current methods for the in vitro derivation of T cells from murine and human HSPCs and hPSCs that use feeder‐cell and feeder‐cell‐free systems. Furthermore, we explore their potential for adoption for use in T‐cell‐based therapies. S tem C ells 2015;33:3174–3180