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Evaluation of a new multiple‐layer spotting technique for matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry of synthetic polymers
Author(s) -
Meier Michael A. R.,
Schubert Ulrich S.
Publication year - 2003
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.961
Subject(s) - chemistry , mass spectrometry , sample preparation , analyte , time of flight mass spectrometry , matrix assisted laser desorption/ionization , polymer , desorption , matrix (chemical analysis) , chromatography , analytical chemistry (journal) , resolution (logic) , ambient ionization , ionization , adsorption , ion , chemical ionization , organic chemistry , artificial intelligence , computer science
Abstract A new multiple‐layer matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) sample‐spotting technique is described. This fast and easy technique was evaluated with poly(ethylene glycol) (PEG) standards and optimized conditions for these synthetic polymers were obtained. PEGs up to 35 kDa were detectable with this approach and single monomer resolution was observed up to 20 kDa. The spotting was performed using a multiple‐layer approach, which offers the capability of complex sample preparation without the requirement of premixing the different matrix, analyte and doping salt solutions. The technique reduces the time required for sample preparation and offers high flexibility with respect to sample composition and solvents utilized for the crystallization of the compounds. The technique is thus perfectly suited for applications in combinatorial material research. Copyright © 2003 John Wiley & Sons, Ltd.