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Structural determination of hexadecanoic lysophosphatidylcholine regioisomers by fast atom bombardment tandem mass spectrometry
Author(s) -
Hong Jongki,
Kim Young Hwan,
Gil Ji Hye,
Cho Kun,
Jung Jee H.,
Han SoYeop
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.835
Subject(s) - chemistry , structural isomer , tandem mass spectrometry , protonation , fast atom bombardment , lysophosphatidylcholine , mass spectrometry , ion , molecule , collision induced dissociation , dissociation (chemistry) , analytical chemistry (journal) , mass spectrum , tandem , chromatography , stereochemistry , organic chemistry , phospholipid , biochemistry , materials science , membrane , composite material , phosphatidylcholine
The structural determination of sn ‐1 and sn ‐2 hexadecanoic lysophosphatidylcholine (LPC) regioisomers was carried out using fast atom bombardment tandem mass spectrometry (FAB‐MS/MS). The collision‐induced dissociation (CID) of protonated and sodiated molecules produced diverse product ions due mainly to charge remote fragmentations. Based on the information obtained from the CID spectra of protonated and sodiated molecules, sn‐1 and sn‐2 hexadecanoic LPC isomers could be discriminated. Especially, the abundance ratio of the diagnostic ion pair [ m/z 224/226] in the CID spectra of [M + H] + ions was shown to be greatly different. Moreover, the CID‐MS/MS spectra of sodium‐adducted molecules for hexadecanoic LPC isomers showed characteristic product ions such as [M + Na − 103] + , [M + Na − 85] + , and [M + Na − 59] + , by which their regio‐specificity can be differentiated. Copyright © 2002 John Wiley & Sons, Ltd.
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