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Mass spectrometric analysis of cyclofenil and its metabolites in human urine
Author(s) -
Myung SeungWoon,
Min HyeKi,
Kim DongHyun,
Kim Myungsoo,
Cha SuJin,
Yoo EunAh,
Yim YongHyeon
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.636
Subject(s) - chemistry , chromatography , glucuronide , urine , mass spectrometry , thermospray , high performance liquid chromatography , chemical ionization , trimethylsilyl , hydrolysis , gas chromatography–mass spectrometry , tandem mass spectrometry , metabolite , atmospheric pressure chemical ionization , conjugate , gas chromatography , selected reaction monitoring , ionization , organic chemistry , biochemistry , ion , mathematical analysis , mathematics
Using gas chromatography/electron impact‐mass spectrometry (GC/EI‐MS) and high performance liquid chromatography with atmospheric pressure chemical ionization tandem mass spectrometry (HPLC/APCI‐MS/MS), the structures of cyclofenil metabolites in human urine have been assigned. The hydroxyl metabolites liberated from the glucuronide conjugates after acid hydrolysis were characterized as the trimethylsilyl (O‐TMS) derivatives using GC/MS. The conjugate glucuronide forms were detected without hydrolysis by HPLC/MS. Cyclofenil was not observed in urine. Tentative structures for the two metabolites are proposed. Copyright © 2002 John Wiley & Sons, Ltd.
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