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Urinary 19‐norandrosterone purification by immunoaffinity chromatography: application to gas chromatography/combustion/isotope ratio mass spectrometric analysis
Author(s) -
Desroches M. C.,
Mathurin J. C.,
Richard Y.,
Delahaut P.,
de Ceaurriz J.
Publication year - 2002
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.584
Subject(s) - chemistry , chromatography , gas chromatography , mass spectrometry , extraction (chemistry) , combustion , isotope ratio mass spectrometry , column chromatography , urine , biochemistry , organic chemistry
Abstract The detection of exogenous 19‐norandrosterone (19‐NA) in urines was investigated by using gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS). 19‐NA is, for the first time to our knowledge, isolated from urinary matrix by specific immunoaffinity chomatography (IAC) before analysis. The sample preparation consisted of a preliminary purification of urine by solid‐phase extraction after hydrolysis by β‐glucuronidase. Unconjugated 19‐NA was thus isolated by IAC and directly analysed by GC/C/IRMS. Optimisation of IAC purification was achieved and the reliability of the technique for anti‐doping control is discussed. Copyright © 2002 John Wiley & Sons, Ltd.