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Effects of luteolin on the inhibition of proliferation and induction of apoptosis in human myeloid leukaemia cells
Author(s) -
Ko W. G.,
Kang T. H.,
Lee S. J.,
Kim Y. C.,
Lee B. H.
Publication year - 2002
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.871
Subject(s) - luteolin , apoptosis , dna fragmentation , programmed cell death , agarose gel electrophoresis , cell growth , flavonoid , biology , growth inhibition , fragmentation (computing) , flow cytometry , microbiology and biotechnology , biochemistry , dna , antioxidant , ecology
Abstract Luteolin, a flavonoid isolated from the fruit of Vitex rotundifolia , has been examined with regard to the inhibition of proliferation and induction of apoptosis in human myeloid leukaemia HL‐60 cells. The concentration required for 50% inhibition of the growth after 96 h was 15 ± 1.1 μ M . The mode of cell death induced by luteolin was found to be apoptosis, as judged by the morphologic alteration of the cells and by the detection of DNA fragmentation using agarose gel electrophoresis. The degree of apoptosis was quantified by a sandwich enzyme immunoassay and flow cytometric analysis. These results suggest that luteolin may be used as potential chemopreventive and chemotherapeutic agents. Copyright © 2002 John Wiley & Sons, Ltd.