Premium
Inhibition of cyclooxygenase (COX) enzymes by compounds from Daucus carota L. Seeds
Author(s) -
Momin Rafikali A.,
De Witt David L.,
Nair Muraleedharan G.
Publication year - 2003
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.1296
Subject(s) - cyclooxygenase , naproxen , chemistry , enzyme , ibuprofen , prostaglandin , aspirin , enzyme inhibitor , biochemistry , pharmacology , biology , medicine , alternative medicine , pathology
Abstract Cyclooxygenase (COX) enzymes inhibitory assay directed investigation of Daucus carota seed extracts resulted in the isolation and characterization of compounds, 2,4,5‐trimethoxybenzaldehyde (1), oleic acid (2), trans ‐asarone (3) and geraniol (4). Compounds 1–4 showed 3.32, 45.32, 46.15, and 3.15% of prostaglandin H endoperoxide synthase‐I (COX‐I) inhibitory activity and 52.69, 68.41, 64.39 and 0% prostaglandin H endoperoxide synthase‐II (COX‐II) inhibitory activity, respectively at 100 mg mL −1 . Compound 1 showed selectivity towards COX‐II enzyme inhibition at 100 µg mL −1 . The COX‐II/COX‐I ratio for compound 1 was 17.68 at 100 µg mL −1 compared to solvent control. Ibuprofen, Naproxen, Aspirin, Celebrex and Vioxx at concentrations of 2.06, 2.52, 180, 1.67 and 1.67 µg mL −1 , respectively, gave COX‐II/COX‐I ratios of 1.13, 0.92, 0.24, 16 and 75, respectively. The inhibition of COX‐II enzymes by compounds 1 at 100 µg mL −1 was signicant when compared to Aspirin, Ibuprofen, Naproxen and Celebrex at concentrations studied. Copyright © 2003 John Wiley & Sons, Ltd.