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Tat cell‐penetrating peptide has the characteristics of a poly(proline) II helix in aqueous solution and in SDS micelles
Author(s) -
Ruzza Paolo,
Calderan Andrea,
Guiotto Andrea,
Osler Alessio,
Borin Gianfranco
Publication year - 2004
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.558
Subject(s) - aqueous solution , peptide , micelle , cell penetrating peptide , chemistry , proline , membrane , helix (gastropod) , biophysics , cell , molecule , cell membrane , biochemistry , amino acid , organic chemistry , biology , ecology , snail
Abstract Tat cell‐penetrating peptide (GRKKRRQRRRPPQG) is able to translocate and carry molecules across cell membranes. Using CD spectroscopy the conformation of this synthetic peptide was studied in aqueous and membrane‐mimicking, micellar SDS solutions at different temperatures. The CD spectrum of the Tat cell‐penetrating peptide in SDS micellar solution was virtually unchanged from that in aqueous solution, and at low temperature it was close to that of a poly(proline) II helix. Copyright © 2004 European Peptide Society and John Wiley & Sons, Ltd.