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Genome‐wide identification and analysis of genes associated with RNA interference in Bemisia tabaci
Author(s) -
Tian Lixia,
Zeng Yang,
Xie Wen,
Wu Qingjun,
Wang Shaoli,
Zhou Xuguo,
Zhang Youjun
Publication year - 2019
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.5415
Subject(s) - rna interference , biology , gene knockdown , gene , gene silencing , genetics , rna silencing , gene expression , computational biology , genome , pest analysis , rna , botany
ABSTRACT Background As a method of RNA‐mediated gene silencing, RNA interference (RNAi) is a useful reverse genetic tool with which to study gene function, and holds great promise for pest management. Bemisia tabaci is a cosmopolitan pest that causes extensive damage to crops. The mechanism underlying RNAi efficiency in B. tabaci is not well known. We identified and analyzed candidate genes in the RNAi pathway to understand the RNAi mechanism and provide a basis for the application of RNAi in pest management. Results We identified 33 genes putatively involved in the RNAi pathway from the B. tabaci Q genome. Phylogenetic and structural analyses confirmed the characteristics of these genes. Furthermore, quantitative real‐time reverse transcription polymerase chain reaction (qRT‐PCR) and transcriptomic analysis profiled gene expression patterns during different developmental stages. Gene expression levels estimated by qRT‐PCR and RNA‐seq analyses were significantly correlated. Moreover, gene functions were verified by RNAi. When accompanied by knockdown of AGO2 , Dicer2 and Sid1 , the efficiency of CYP6DB3 RNAi decreased correspondingly. Conclusion In this study, we annotated and validated genes involved in B. tabaci RNAi. A better understanding of the building blocks of the RNAi process in B. tabaci facilitates integration of this novel biotechnology into the management of this emerging pest, either directly or indirectly. © 2019 Society of Chemical Industry