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Differential expression of matrix metalloproteinases and their tissue inhibitors in human primary cultured prostatic cells and malignant prostate cell lines
Author(s) -
Zhang Ju,
Jung Klaus,
Lein Michael,
Kristiansen Glen,
Rudolph Birgit,
Hauptmann Steffen,
Schnorr Dietmar,
Loening Stefan A.,
Lichtinghagen Ralf
Publication year - 2001
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.10030
Subject(s) - stromal cell , lncap , matrix metalloproteinase , cell culture , prostate , biology , cell , pathology , cancer research , chemistry , medicine , cancer , biochemistry , genetics
Abstract BACKGROUND The aim was to investigate the expression of matrix metalloproteinases (MMPs), membrane type MMPs (MT‐MMPs), and their inhibitors (TIMPs) in human primary cultured prostatic cells and malignant prostate cell lines. METHODS Reverse transscription‐polymerase chain reaction‐based measurements of the mRNA levels of MMP‐2, MMP‐7, MT1‐MMP, MT3‐MMP, TIMP‐1, and TIMP‐2 in relation to the house‐keeping gene glyceraldehyde phosphate dehydrogenase were performed in cancerous and non‐cancerous prostatic tissue samples, in primary cell cultures of epithelial cells, in both fibroblasts, and smooth‐muscle cells as stromal cells, and in the human malignant prostatic cell lines DU‐145, LNCaP, and PC‐3. RESULTS MMP‐2 was mainly expressed in the stromal cells and MMP‐7 showed their highest values in the epithelial cells. MT1‐MMP, MT3‐MMP, TIMP‐1, and TIMP‐2 were found both in the stromal and in the epithelial cells, but there were some differences between the expressions in fibroblasts and smooth‐muscle cells. Different expressions were also observed between the cells deriving from the primary cell cultures, the benign cell line BPH‐1, and the malignant cell lines LNCaP, D‐145, and PC‐3. CONCLUSIONS These exemplary results concerning different expressions of MMPs and TIMPs in cells from prostatic tissue suggest that a better insight into changes observed in prostatic tissue needs studies on cells cultured from the tissue. Prostate 50: 38–45, 2002. © 2002 Wiley‐Liss, Inc.