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Structures of a pan‐specific antagonist antibody complexed to different isoforms of TGFβ reveal structural plasticity of antibody–antigen interactions
Author(s) -
Moulin Aaron,
Mathieu Magali,
Lawrence Catherine,
Bigelow Russell,
Levine Mark,
Hamel Christine,
Marquette JeanPiere,
Parc Josiane,
Loux Christophe,
Ferrari Paul,
Capdevila Cecile,
Dumas Jacques,
Dumas Bruno,
Rak Alexey,
Bird Julie,
Qiu Huawei,
Pan Clark Q.,
Edmunds Tim,
Wei Ronnie R.
Publication year - 2014
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1002/pro.2548
Subject(s) - gene isoform , antibody , antigen , chemistry , transforming growth factor , microbiology and biotechnology , computational biology , biology , immunology , biochemistry , gene
Abstract Various important biological pathways are modulated by TGFβ isoforms; as such they are potential targets for therapeutic intervention. Fresolimumab, also known as GC1008, is a pan‐TGFβ neutralizing antibody that has been tested clinically for several indications including an ongoing trial for focal segmental glomerulosclerosis. The structure of the antigen‐binding fragment of fresolimumab (GC1008 Fab) in complex with TGFβ3 has been reported previously, but the structural capacity of fresolimumab to accommodate tight interactions with TGFβ1 and TGFβ2 was insufficiently understood. We report the crystal structure of the single‐chain variable fragment of fresolimumab (GC1008 scFv) in complex with target TGFβ1 to a resolution of 3.00 Å and the crystal structure of GC1008 Fab in complex with TGFβ2 to 2.83 Å. The structures provide further insight into the details of TGFβ recognition by fresolimumab, give a clear indication of the determinants of fresolimumab pan‐specificity and provide potential starting points for the development of isoform‐specific antibodies using a fresolimumab scaffold.