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Quantification of Calcyclin and Heat Shock Protein 90 in Sera from Women with and without Preeclampsia by Mass Spectrometry
Author(s) -
Güzel Coşkun,
den Berg Caroline B.,
Duvekot Johannes J.,
Stingl Christoph,
den Bosch Thierry P. P.,
der Weiden Marcel,
Steegers Eric A. P.,
SteegersTheunissen Regine P. M.,
Luider Theo M.
Publication year - 2019
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201800181
Subject(s) - preeclampsia , heat shock protein , pregnancy , shock (circulatory) , pathogenesis , medicine , hsp70 , endocrinology , first trimester , andrology , chemistry , fetus , biology , biochemistry , gene , genetics
Purpose The objective of present study is to determine serum levels and placental distribution of two interacting proteins calcyclin and heat shock protein 90 in preeclampsia. Experimental design Maternal serum levels of calcyclin and heat shock protein 90 are compared throughout pregnancy from the first trimester till term among women with preeclampsia ( n = 43) and age‐matched normotensive pregnant controls ( n = 46). A serum‐based 2D LC‐MS assay using Parallel Reaction Monitoring is applied to quantify both calcyclin and heat shock protein 90. Results Serum levels of calcyclin are significantly lower in patients with preeclampsia in the second trimester of pregnancy as compared to controls ( p < 0.05). Serum levels of heat shock protein 90 are significantly higher in patients with preeclampsia in the third trimester as compared to controls ( p < 0.001). Conclusion and clinical relevance Both interacting proteins calcyclin and heat shock protein 90 are notably changed in preeclamptic patients compared to controls. Calcyclin is already decreased before the onset of preeclampsia in the second trimester and HSP90 is strongly increased in the third trimester. This suggests that these proteins may play a role in the pathogenesis of preeclampsia and ought to be investigated in large cohort studies as molecular biomarkers.